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Peptide ligation

手順3:SEAペプチドを用いたNatural Chemical Ligation 脱気したライゲーション反応緩衝液(0.1Mリン酸塩緩衝液、0.2M 4-メルカプトフェニル酢酸(MPAA)、0.2M TCEP*HCl、pH7.4)を含むチューブに不活性雰囲気下で酸化されたSEAペプチド(1等量)とN-末端Cysペプチド(1.5等量)を溶解させます namely the 'peptide ligation' approach [4,6]. Peptide lig- ation actually refers to a suite of approaches, all of which allow fully unprotected polypeptide building blocks to be regioselectively joined together in aqueous solution to create a target protein molecule. Ligation can be achieved either chemically, by incorporating uniqu Orthogonal peptide ligation is an amino terminal specific method to couple chemically unprotected peptides or proteins derived from synthetic or biosynthetic sources Two essential features enable peptide ligation in water: the reactivity and p KaH of α-aminonitriles makes them compatible with ligation at neutral pH and N -acylation stabilizes the peptide..

Chemical protein synthesis (CPS) that consists of solid-phase peptide synthesis and peptide ligation generates not only naturally-occurring proteins with/without posttranslational modifications (PTMs), but also a variety of artificial proteins including unnatural amino-acids such as ᴅ-amino acids and fluorophore-labeled amino acids The synthesis of proteins requires efficient native peptide ligation methods, which enable the chemoselective formation of a native peptide bond in aqueous solution between unprotected peptide segments. The most frequently used technique for synthesizing proteins is Native chemical ligation (NCL)

化学ライゲーション (かがくライゲーション、 英: chemical ligation )は、長鎖の ペプチド あるいは タンパク質 を作るために用いられる一連の技術である。 収束的合成 の第2段階である Chemical ligation is a set of techniques used for creating long peptide or protein chains. It is the second step of a convergent approach. First, smaller peptides containing 30-50 amino acids are prepared by conventional chemical peptide synthesis. Then, they are completely deprotected この壁を克服する方法論として有効なのが、 Native Chemical Ligation (NCL) 法 である。. C末端にチオエステルを持つペプチドとN末端に無保護システインを持つペプチドを、生体適合条件下 (pH 7、20℃~37℃)に混ぜるだけで反応が進行し、余分な活性化剤を必要とし.

Methods and strategies of peptide ligation. *. James P. Tam. Corresponding Author. tamjp@ctrvax.vanderbilt.edu. Department of Microbiology and Immunology, Vanderbilt University, A5119 MCN, Nashville, TN 37232. Department of Microbiology and Immunology, Vanderbilt University, A5119 MCN, Nashville, TN 37232 Search for more papers by this author Enzyme-catalyzed peptide ligation is a powerful tool for site-specific protein bioconjugation, but stringent enzyme-substrate specificity limits its utility. We developed an approach for.. Chemical and enzymatic approaches to peptide ligation are important alternatives of recombinant DNA technology for protein synthesis and modification. Although as old as that of chemical procedures, enzyme-mediated peptide ligation is far less developed than that of chemical counterpart due to the difficult availability of peptide ligase Peptide ligation chemistry at selenol amino acids ‡ Lara R. Malins School of Chemistry, The University of Sydney, Sydney, NSW, 2006 Australia These authors contributed equally to this review Search for more papers by this. Peptide ligation is an indispensable step in the chemical synthesis of target peptides and proteins that are difficult to synthesize at once by a solid-phase synthesis. The ligation reaction is generally conducted with two peptide fragments at a high aqueous concentration to increase the reaction ra

Peptide-peptide ligation enabled covalent and site-specific polymerization of affibodies or antibodies against the tumor markers epidermal growth factor receptor (EGFR) and HER2 ligation of a peptide to an oligonucleotide, the peptide being presented by a second oligonucleotide in the form a reactive thioester-linked intermediate. The reaction scheme is illustrated in Figure 1. As native tected peptide that. 詳細の表示を試みましたが、サイトのオーナーによって制限されているため表示できません

Intermolecular radical 1,2-addition (IRA) of N-tert-butyl-N-(1-diethylphosphono-2,2-dimethylpropyl)aminoxyl (SG1) based alkoxyamines onto activated olefins is used as a tool for peptide ligation. This strategy relies on. Native chemical ligation has been the leading methodology for synthetic access to complex proteins. The method involves an attack by the thiol group of an N-terminal cysteine residue of one peptide on a C-terminal thioester of CPE peptide ligation using a building block containing a cysteinyl prolyl ester (CPE) autoactivating unit is described. The peptide building block can be prepared by a standard Fmoc chemistry and used in thiol-mediated ligation, such as native chemical ligation A fluorogenic native chemical ligation for assessing the role of distance in peptide-templated peptide ligation. Henrik Petszulat, Oliver Seitz. Pages 5022-5030. Download PDF. Article preview. Graphical abstract. Graphical abstract. ISSN: 0968-0896

ライゲーション反応 株式会社ビジコムジャパ

Peptide Ligases. Enzyme-catalyzed peptide ligation is a powerful tool that enables the assembly of peptides, proteins and protein conjugates. It is carried out by enzymes known as 'peptide ligases' which catalyze the formation of peptide bonds with site and substrate specificity. These peptide-bond staplers enable not only site-specific. An efficient method has been developed for the salicylaldehyde ester-mediated ligation of unprotected peptides at serine (Ser) or threonine (Thr) residues. The utility of this peptide ligation approach has been demonstrated through the convergent syntheses of two therapeutic peptides--ovine-corticoliberin and Forteo--and the human erythrocyte acylphosphatase protein (∼11 kDa). The. With this method, the side chain-to-side chain cyclic peptide, branched/bridged peptides, tailed cyclic peptides and multi-cyclic peptides have been designed and successfully synthesized with native peptidic linkages at the ligation sites. This strategy has provided an alternative strategic opportunity for synthetic peptide development Researchers in Hong Kong and Germany have unveiled a new chemical ligation strategy to design peptides with a range of complex structural features. The group, led by Xuechen Li at the University. Durch die Proteinligation können zwei Peptide aneinander gekoppelt werden. Meist handelt es sich dabei um Peptidfragmente, die durch Festphasenpeptidsynthese (SPPS) hergestellt wurden. Verschiedene Verfahren zur Proteinligation wurden beschrieben, z. B.: die native chemical ligation

  1. al thioacid or thioester and an N-ter
  2. Peptide Ligation United States Patent Application 20160122383 Kind Code: A1 Abstract: The invention relates to a process for introducing a thiol group a to a carbonyl group in a side Inventors: Payne, Richard J. (Camperdown.
  3. al cysteine (Cys) residue and is mediated by the nucleophilic Cys thiol functionality. A widely adopted extension of the technique for the.
  4. Browse the Products offered by NEB for Peptide Ligation Are you doing COVID-19 related research? Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes..
  5. 1. Chan, W. C., White, P. D., Fmoc Solid Phase Peptide Synthesis: A Practical Approach, New York: Oxford University Press, 2000. Google Scholar Affiliations.
  6. Ligation is assisted by templating peptide nucleic acid (PNA) strands, and therefore, ligation specificity is solely encoded by the PNA sequence. PNA templating, in general, allows for various kinds of covalent ligation reactions. A
  7. メリフィールド ペプチド固相合成法 Merrifield Solid-Phase Peptide Synthesis ネイティブ・ケミカル・リゲーション Native Chemical Ligation (NCL) 縮合剤 Condensation Reagent アザ-ウィティッヒ反応 Aza-Wittig Reaction ウギ反応 Ug

Methods and strategies of peptide ligatio

Chemical Protein Synthesis by Kinetically Controlled Ligation of Peptide O-Esters Ji-Shen Zheng , Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084 (China), Fax: (+86) 10-6277114 Peptide ligation strategies utilize three steps (Figure 2). An initial capture step links the peptides by a chemical reaction that is more rapid than intermolecular acyl transfer to an amine and that uses functional groups with reactivity. chemical ligation originated by Kent's group in 1994 is one of the most widely used synthetic methods. 1 It leads to a native cysteine amide bond by reacting one peptide bearing a carboxylic end with the other having a cysteine terminal CPE peptide ligation using a building block containing a cysteinyl prolyl ester (CPE) autoactivating unit is described. The peptide building block can be prepared by a standard Fmoc chemistry and used in thiol-mediated ligation, suc

Peptide ligation by chemoselective aminonitrile coupling in

  1. Oxime ligation represents a class of biocompatible click chemistry that allows the structural diversity of libraries of aldehydes to be rapidly evaluated within the context of a parent oxime‐containing peptide platform. Importantly, oxime ligation represents a form of post solid‐phase diversification, which provides a facile and
  2. o acid to the resin, coupling strategies in stepwise peptide chain elongation, and approaches to.
  3. Post-translational modifications of histones influence both chromatin structure and the binding and function of chromatin-associated proteins. A major limitation to understanding these effects has been the inability to construct nucleosomes in vitro that harbor homogeneous and site-specific histone modifications. Here, we describe a native peptide ligation strategy for generating nucleosomal.
  4. Peptide-protein interactions are corner-stones of living functions involved in essential mechanisms, such as cell signaling. Given the difficulty of obtaining direct experimental structural biology data, prediction of those interactions is of crucial interest for the rational development of new drugs, notably to fight diseases, such as cancer or Alzheimer's disease. Because of the.

タンパク質化学合成を加速するペプチドライゲーションの新技

Sulfide-mediated peptide synthesis by N-to-C terminal ligation of aminonitriles in water. All 20 proteinogenic side chain residues were tolerated (and gave high yields) in amidothioacid ligation, including histidine, aspartate, cysteine, serine, threonine, and tyrosine, which are essential to enzyme catalysis but are notoriously difficult to couple by previously reported (prebiotic) peptide. The ligation reaction of unprotected peptide fragments proceeds in aqueous media (neutral to slightly basic pH) at ambient temperature with extremely high average ligation yields (up to 98% in <1 h). Only a low molar excess of acyl acceptor (1.1-2 molar equivalents) is required ( Schmidt et al., 2017b ) This approach would offer a convergent ligation toward large peptides in sharp contrast to tedious traditional iterative peptide syntheses. Thus, a plethora of di- and tripeptides were efficiently synthesized in an atom-economical manner under exceedingly mild reaction conditions that did not jeopardize the tolerance of sensitive functional groups on the side chain of amino acids ( Fig. 2 ) chemical ligation, are important reactions in Foden et al., Science 370, 865-869 (2020) 13 November 2020 2of5 Prebiotic synthesis of cysteine peptides that catalyze peptide ligation in neutral wate Peptide carboxy terminal thioesters are used as the activated intermediate in nonribosomal peptide synthesis and peptide ligation step in protein splicing ( 48, 49). In addition to modern biological reactions, peptide and amino-acid thioesters have been proposed to participate in the prebiotic world because they can be utilized for nonenzymatic synthesis of primitive polypeptides

% Researchers in Hong Kong and Germany have unveiled a brand new chemical ligation technique to design peptides with a spread of complicated structural Welcome! Log into your accoun INTRODUCTION The native chemical ligation concept was first developed by Dawson et al. in 1994, in which one protein or peptide with a C-terminal thioester and the other with a N-terminal cysteine selectively undergo thiol-thioester exchange and then S-to-N acyl transfer to form a larger protein or peptide (Figure 1A) 1 Peptide ligation from alkoxyamine based radical addition† Thomas Trimaille,*a Laurent Autissier,a Mamy Daniel Rakotonirina,a Yohann Guillaneuf,a Claude Villard,b Denis Bertin,a Didier Gigmesa and Kamel Mabrouk*a DOI: 1 ligation appeared to be slower than that of peptide-peptide ligation, Figure 1. Sortase-mediated ligation of RE (Edans)LPKTGK(Dabcyl)R with GnPep (n ) 1, 2, 3, or 5): (A) Fluorescence measurement of sortase cleavage rate of 0.1 A new thiol additive for one-pot sequential peptide ligation-desulfurization chemistry ポスター発表 優秀賞 日本ペプチド学会が授与する賞です。 演題番号 受賞者氏名(所属) 演題 P-007 加茂 直己 (東京大) Total chemical P-060.

Contact. Peptide Trends January 2020. January 30, 2020. MEET US AT THE IBERIAN PEPTIDE MEETING. Join us at the 17 th Iberian Peptide Meeting to be held in Madrid, Spain, on February 5-7, 2020. Bachem supports its customers in the pursuit of groundbreaking discoveries that further scientific advances, particularly in the field of medicine An enigmatic peptide ligation reaction: Protease-catalyzed oligomerization of a native protein segment in neat aqueous solution SANGARALINGAM KUMARAN, DEBJANI DATTA, and RAJENDRA P. ROY National Institute o This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold. The KAHA ligation employs C-terminal peptide α-ketoacids (KAs) and N-terminal peptide hydroxylamines (HAs), which react chemoselectively to form amides or esters. This reaction proceeds in aqueous media without reagents or catalysts. By developing new building blocks and chemical reactions, we can now easily prepare large peptide segments (40.

SEA Native Peptide Ligation - Wikipedi

Other Staudinger ligation induced macrocyclizations have been published previously by Maarseveen and co-workers, who successfully used the Raines ligation reagent for the synthesis of a series of medium-sized lactams. 4 Wong and co-workers reported the synthesis of 14 different glycopeptides through the traceless Staudinger Ligation. 5 For this work, they also developed a protease-catalyzed. キーワード が『Peptide ligation』と一致する講演:1件該当しました。 検索結果は開始時刻順に並んでいます。また、フラッシュ付きポスターの場合、フラッシュとポスターの2件となります Abstract: Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester This designed RNA can also accelerate the peptide ligation. The resulting ligated peptide, which has two RNA-binding sites, can in turn function as a trans -acting factor that enhances the endonuclease activity catalyzed by th

Researchers in Hong Kong and Germany have unveiled a new chemical ligation strategy to design peptides with a range of complex structural features. The group, led by Xuechen Li at the University of Hong Kong, used benzofuran groups to incorporate salicylaldehyde esters in the peptide side chains, which enabled them to construct a diverse array of peptide scaffolds The peptide thioester is used as a building block for protein synthesis. In this research, we developed new methods for the peptide thioester synthesis. Based on the structure of Cys-Pro ester(CPE), Cys-Pro-Cys sequence was found to produce a peptide thioester. It could be prepared as a recombinant protein. When N-substitute Gly residue is introduced instead of the Pro residue in the CPE.

化学ライゲーション - Wikipedi

This Staudinger Peptide Ligation proceeds via formation of a 7-membered transition state to afford di-, tetra- and pentapeptides in 78-95% yields. The experimental results of ligation at Gly. Reagents for Biomolecular Peptide Conjugation The Novabiochem® brand of Merck offers reagents for peptide modification for biomolecule conjugation via oxime, thiol-ene, and hydrazone ligation. 答えをローディング SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. by Jacob O Fierer, Gianluca Veggiani, Mark Howarth. Proceedings of the National Academy of Sciences of the United States o

Chemical ligation - Wikipedi

  1. o acids is feasible, 8 larger peptides and small proteins, as yet, are not accessible by solid phase peptide synthesis alone. . Much larger products can be assembled by coupling protected peptide segments in so
  2. ligation step.Multistep NCL of different peptide-segments, however, can lead to larger proteins [11].An extension of this NCL strategy is the expressed protein ligation (EPL
  3. Chisholm, TS, Kulkarni, SS, Hossain, KR, Cornelius, F, Clarke, RJ & Payne, RJ 2020, ' Peptide Ligation at High Dilution via Reductive Diselenide-Selenoester Ligation.
  4. SEA Native Peptide Ligation and related information | Frankensaurus.com helping you find ideas, people, places and things to other similar topics. Medication used to treat low sexual desire in women. Used for low sexual desire.

Convergent chemical synthesis of proteins by ligation of peptide hydrazides. Angew. Chem. Int. Ed. 2012, 51, 10347-10350. Genetically encoded alkenyl-pyrrolysine analogues for thiol-ene reaction mediated site-specific protein Article HF-Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization Detailed information of the J-GLOBAL is a service based on the concept of Linking, Expanding, and Sparking, linking science and technology. Native chemical ligation Native chemical ligation (NCL) is a convergent synthetic strategy based on the linear coupling of glycopeptide fragments. This technique makes use of the chemoselective reaction between a N-terminal cysteine residue on one peptide fragment with a thio-ester on the C-terminus of the other peptide fragment [19] as illustrated below

Automated Nucleic Acid Extraction Manufacturers, Factory, Suppliers From China, We sincerely hope to provide you and your company with a great start. If there is anything we will do to suit your needs, we shall be additional than. The IPL reaction allows the ligation of a synthetic peptide or a protein with an N-terminal cysteine residue to the thioester on the C-terminus of an expressed protein through a native peptide bond (1). The IPL protocol employs the.

Peptide ligases are enzymes that naturally function as both molecular scissors and glue for multiple protein engineering applications. Our suite of plant-derived peptide ligases are made in the laboratory, and can be used for multiple applications, including: Stabilising pharmaceuticals by creating circular peptides Peptide ligation strategies which enable the combined use of the chemical synthesis and DNA-based recombinant technology would be a robust tool in biological research and greatly expand the scope of applications. 1,2 The current ligation

ネイティブ・ケミカル・ライゲーション Native Chemical Ligation

  1. 図5 糖タンパク質合成における可能な2つのルート. ).ルート1では,固相合成の際,糖鎖をもつアミノ酸を導入して糖ペプチドチオエステルを合成し,ライゲーションとフォールディングをへて糖タンパク質を得る.一方,ルート2では,還元末端糖のみを.
  2. unreactive peptide and peptide ligation by the RBM strategy. Angewandte Forschungsartikel Chemie Angew.Chem. 2019, 131,12359-12365 T 2019 Wiley-VCH Verlag GmbH &Co. KGaA,Weinheim www.angewandte.de 12361 ,.
  3. A Native Peptide Ligation Strategy for Deciphering Nucleosomal Histone Modifications* Received for publication, February 10, 2003, and in revised form, February 19, 2003 Published, JBC Papers in Press, February 20, 2003, DOI 1
  4. Staudinger Ligation: A Peptide from a Thioester and Azide Bradley L. Nilsson,† Laura L. Kiessling,†,‡ and Ronald T. Raines*,†,‡ Departments of Chemistry and Biochemistry, UniVersity of Wisconsin-Madison, Madison, Wisconsi
  5. 近接ライゲーションアッセイ(PLA:Proximity Ligation Assay)は、ELISAの特異性とPCRの感度を組み合わせた、均質で高特異性、高感度の免疫組織化学的(英語)ツールです。 この技術は、2002年にFredrikssonらによっ.
  6. o acid for non-racemization coupling, gave >99.8% pure peptide using his peptide synthesis. Furthermore, his new method provides a new peptide chemical ligation procedure for any a

SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture Jacob O. Fierer1, Gianluca Veggiani1, and Mark Howarth2 Department of Biochemistry, University of Oxford, Oxford, OX1 3Q Oxime ligation represents a class of biocompatible click chemistry that allows the structural diversity of libraries of aldehydes to be rapidly evaluated within the context of a parent oxime-containing peptide platform. Importantl The Kent research group pioneered the effective total chemical synthesis of protein molecules. The long polypeptide chain of the protein molecule is prepared in a convergent fashion by 'chemical ligation', the chemoselective condensation of unprotected peptide segments that contain unique, mutually reactive functional groups peptide segments, the two subunits were ligated to form a new peptide bond. Native chemical ligation extended the concept by creating a native peptide bond and providing new approaches to protein engineering. Keywords: 1.

Traceless Staudinger Ligation | Sigma-Aldrich

Peptide ligation strategies, such as the thioester method (11, 12) and native chemical ligation (NCL) (), are widely used in protein synthesis. We previously reported on the total chemical synthesis of histone H3 containing a N ε -trimethylated lysine residue at the 9 position by sequential ligations employing NCL followed by the thioester method ( 14 ) peptide ligation which was first reported in 2000 by Nilsson et al.9 There the original process9 started from an alkyl thi-oester of a peptide which was converted by a transthiola-tion reaction with o-(diphenylphosphino)-benzenethiol 6.

Methods and strategies of peptide ligation* - Tam - 2001

Methods and strategies of peptide ligation*. James P. Tam Jiaxi Xu Khee Dong Eom Methods and Strategies of Department of Microbiology Peptide Ligation* and Immunology, Vanderbilt University, A5119 MCN, Nashville, TN 37232 Abstract: This review focuses on the concept, methods, and strategies of orthogonal peptide ligation Keywords: peptide ligation, solid phase peptide synthesis, protein synthesis, chemoselectivity, peptide synthesis Citation: Wong CTT, Li T, Lam HY, Zhang Y and Li X (2014) Realizing serine/threonine ligation: scope and. Read Peptide ligation chemistry at selenol amino acids, Journal of Peptide Science on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at you Peptide Ligation Using a Photoremovable Auxiliary 元データ 2004-03-01 著者 Kawakami Toru Institute For Protein Research Osaka University AIMOTO Saburo Institute for Protein Research, Osaka University Aimoto Saburo.

Engineering peptide ligase specificity by proteomic

Fig

Recent advances in enzyme-mediated peptide ligation

Phrases contain exact peptide ligation from credible sources EXACT : Chi Zhang, Yizhou Li, Mingda Zhang, and Xiaoyu Li*, DNA-directed formation of peptide bond: a model study towards DNA-programmed peptide ligation Tetrahedron, 2012, 68, 5152-5156 Read Peptide Ligation, ChemInform on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Our policy towards the use of cookies All. Native chemical ligation... in NCL . This reaction , which is called SEA Native Peptide Ligation, is a potential alternative to Native Chemical Ligation .[ • Peptide synthesis • Protein synthesis. Peptide ligation chemistry has revolutionized protein science by providing access to homogeneously modified peptides and proteins. However, lipidated polypeptides and integral membrane proteins - an important class of biomolecules - remain enormously challenging to access synthetically owing to poor aqueous solubility of one or more of the fragments under typical ligation conditions. Herein we. Preclinical validations of [18 F]FPyPEGCBT-c(RGDfK): a 18 F-labelled RGD peptide prepared by ligation of 2-cyanobenzothiazole and 1,2-aminothiol to image angiogenesis Didier J. Colin 1, James A. H. Inkster 2, 1 & 2,

Peptide ligation chemistry at selenol amino acids - Malins

ボーディペプチド合成 Bode Peptide Synthesis | Chem-Station (ケムステ)

Simultaneous and Traceless Ligation of Peptide Fragments

SpyLigase peptide-peptide ligation polymerizes affibodies to

Chemical Protein Synthesis by Kinetically Controlled Ligation of Peptide O-Esters. Published in Chembiochem on March 01, 2010 A new detection method for ligandprotein interactions was constructed using interaction-dependent native chemical ligation and enzyme reconstitution (IDNCL-ER) by employing ribonuclease S. Using a coiled-coil peptide pair as a. Chemical ligation is a set of techniques used for creating long peptide or protein chains. 化学ライゲーション(かがくライゲーション、英: chemical ligation)は、長鎖のペプチドあるいはタンパク質を作るために用いられる一連の技術である Designed RNAs with two peptide-binding units as artificial templates for native chemical ligation of RNA-binding peptides Norimasa Kashiwagi, Kohei Yamashita, Hiroyuki Furuta , Yoshiya Ikawa 研究成果 : Contribution to journal › Article › 査 Peptide-directed Template-directed ligation ASJC Scopus subject areas Ecology, Evolution, Behavior and Systematics Molecular Biology Genetics Access to Document 10.1007/s00239-002-2429-7 Other files and links Link to.

ACS Publications: Chemistry journals, books, and references

  1. Peptide ligation from alkoxyamine based radical addition
  2. Peptide Thioesters for Native Chemical Ligation
  3. Peptide Ligation Using a Building Block Having a Cysteinyl
Integrin Targeted TherapeuticsRecent advances in in vivo applications of intein-mediated